Recombinant DNA
Steve Seddon・2 minutes read
Recombinant DNA involves combining DNA sequences by inserting a gene from one organism into the DNA of another, commonly using bacteria and plasmids. This process allows for the creation of recombinant plasmids, enabling bacteria to produce specific proteins like human growth hormone and insulin by inserting the desired gene alongside an antibiotic resistance gene to identify transformed cells.
Insights
- Recombinant DNA involves combining DNA sequences by inserting genes from one organism into another, commonly bacteria and plasmids, creating recombinant plasmids.
- Adding an antibiotic resistance gene alongside desired genes in gene splicing allows identification of transformed bacteria cells, facilitated by techniques such as heat shocking and chemical encouragement of plasmid uptake.
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Recent questions
What is recombinant DNA?
Recombinant DNA involves combining different DNA sequences by inserting a gene from one organism into the DNA of another, often focusing on bacteria and plasmids.
How are recombinant plasmids created?
Recombinant plasmids are created by cutting the DNA and plasmid with the same restriction enzyme to create matching sticky ends, allowing them to join and form recombinant plasmids.
What are examples of successful gene insertions?
Successful examples of gene insertions include inserting genes like human growth hormone and insulin into plasmids, enabling bacteria to produce these proteins.
How do scientists identify transformed bacteria cells?
Scientists may identify transformed bacteria cells by adding an antibiotic resistance gene alongside the desired gene, achieved through methods like heat shocking and using specific chemicals to encourage plasmid uptake.
What is the purpose of gene splicing?
Gene splicing, such as recombinant DNA technology, allows for the creation of genetically modified organisms by combining DNA sequences from different organisms, often used in research and biotechnology for various applications.
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Summary
00:00
Gene splicing creates recombinant DNA in bacteria.
- Recombinant DNA or gene splicing involves combining different DNA sequences by inserting a gene from one organism into the DNA of another, primarily focusing on bacteria and plasmids.
- The process includes cutting the DNA and plasmid with the same restriction enzyme to create matching sticky ends, allowing them to join and form recombinant plasmids.
- Successful examples include inserting genes like human growth hormone and insulin into plasmids, enabling bacteria to produce these proteins.
- Scientists may also add an antibiotic resistance gene alongside the desired gene to identify transformed bacteria cells, achieved through methods like heat shocking and using specific chemicals to encourage plasmid uptake.
