Microbiology & Parasitology Rationalization (January 22, 2024) | Legend Review Center

Legend Review Center28 minutes read

Dr. Gabrielle Paul Pasquan hosts a session on microbiology and parasitology for 200 participants, announcing the enrollment for the August 2024 Meic Board review that includes details for Manila and other branches. The session covers various topics, from confirmatory procedures to parasite identification methods, and use of specific tests for different bacterial species.

Insights

  • The Delta ala test is crucial for confirming hemophilus species, with X Factor and Ala test providing opposite results, highlighting the importance of specific diagnostic procedures in microbiology.
  • Differentiating between various bacterial species relies on specific tests like the D test for clindamycin resistance and the indol positive test for Proteus vulgaris, showcasing the significance of precise laboratory techniques in identifying pathogens accurately.

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Recent questions

  • What is the best specimen for recovering mycobacteria?

    The first morning sputum specimen is ideal.

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Summary

00:00

Microbiology and Parasitology Session with Dr. Pasquan

  • Dr. Gabrielle Paul Pasquan hosts a session on microbiology and parasitology with close to 200 participants.
  • Enrollment for the August 2024 Meic Board review is open, with details for Manila and other branches being finalized.
  • The Delta ala test for Pines is a confirmatory procedure for identifying hemophilus species, with X Factor and Ala test having opposite results.
  • The best specimen for recovering mycobacteria from sputum is the first morning specimen, requiring only one specimen for Gene expert diagnosis.
  • For monitoring tuberculosis patients, DSSM is used, requiring one specimen for monitoring purposes.
  • Three blood cultures from a cancer patient grew lactose negative motile rods, leading to the identification of the organism as a lactose fermentor.
  • The most likely organism in a scenario involving H2S positive reactions is Proteus vulgaris, distinguished by its indol positive test.
  • The definitive host for Echinococcus granulosus is dogs, while humans can serve as both definitive and intermediate hosts for Taenia solium.
  • The D test is used to determine inducible clindamycin resistance in Staphylococcus and Streptococcus, indicating resistance when exposed to both antibiotics simultaneously.
  • Morganella, Providencia, and Proteus are positive for phenylalanine in diamines, while parasitic organisms most often transmitted sexually include Trichomonas vaginalis.

30:09

Protozoa and Bacteria Detection in Stool

  • Cryptosporidium parvum can be detected in stool specimens using acid-fast staining.
  • Other acid-fast protozoa include Isospora and Cyclospora.
  • Toxic shock syndrome is caused by Staphylococcus aureus and Group A Streptococcus.
  • Paragonimiasis is caused by Paragonimus westermani, leading to cough and hemoptysis.
  • Cryptosporidiosis is associated with watery diarrhea in HIV/AIDS patients.
  • Toxoplasmosis can infect the central nervous system in immunocompromised individuals.
  • Enterobiasis, caused by Enterobius vermicularis, does not cause CNS symptoms.
  • Chlamydia trachomatis culture is appropriate for genital specimens.
  • Isospora belli is detected using modified acid-fast staining.
  • Gram's iodine is used as a mordant in the Gram stain procedure.
  • Primary amebic meningoencephalitis is associated with Naegleria fowleri.
  • Listeria monocytogenes can be differentiated from Group B Streptococcus based on motility and catalase tests.
  • Plasmodium falciparum causes severe malaria symptoms.
  • Cila requires a microaerophilic atmosphere for recovery.
  • Cycloserine-cefoxitin-fructose agar is used to recover Clostridium difficile.
  • Hemophilus influenzae type B is the most common cause of infections in young children.
  • Chocolate agar provides both X Factor and V Factor for the recovery of hemophilus species.

59:13

"Identifying Bacteria with RBCs and Lysine"

  • RBCs X Factor and V Factor are derived from the lce RBS, with 92 indicating C 93 massive hemolysis, black fever, and CNS involvement, associated with Plasmodium falciparum.
  • The identification of a bacterium species is linked to various letter shapes and deep blue metachromatic granules, specifically recognized as the Corini bacterium species.
  • Lysine is utilized to differentiate Staphylococcus from Micrococcus, with Micrococcus being lysine-resistant, while Staphylococcus is lysine-sensitive.
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