Lab 5 - Enzymes
Niki Evans・2 minutes read
Enzymes are protein catalysts that speed up chemical reactions by lowering activation energy, with substrate-specificity and unique 3D shapes determining their function. Factors like pH, temperature, and enzyme concentration affect enzymatic activity, as seen in experiments testing how amylase digests starch under different conditions in well plates with iodine.
Insights
- Enzymes are protein-based catalysts that speed up chemical reactions by lowering activation energy. They are substrate-specific, named after the substrate they act on, and have unique 3D shapes determining their function.
- Enzymatic reactions can be influenced by various factors like pH, temperature, and substrate concentration. Denaturation of proteins, like albumin in egg whites, can impact enzyme activity. Testing enzyme activity using well plates with iodine to detect starch digestion time can reveal the effects of enzyme concentration, pH, and temperature on amylase function.
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Recent questions
What are enzymes made of?
Proteins
How do enzymes increase reaction rates?
Lower activation energy
What are cofactors in enzymes?
Non-protein portions
How do enzymes interact with substrates?
Specific active sites
What influences enzymatic activity?
pH, temperature, concentration
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