ELISA (Enzyme-linked Immunosorbent Assay)
Henrik's Lab・2 minutes read
ELISA is a technique used to detect and quantify proteins, with variants like Direct, Indirect, and Sandwich ELISA. In a Sandwich ELISA example, primary antibodies specific for protein X are coated in wells to measure the presence of the antigen.
Insights
- ELISA, or Enzyme-Linked Immunosorbent Assay, is a versatile technique that employs different variants like Direct, Indirect, and Sandwich ELISA to detect and quantify proteins by using enzyme-coupled antibodies, offering a precise and efficient method for research and diagnostic purposes.
- The Sandwich ELISA method, as exemplified in the text, showcases the specificity and sensitivity of this technique by utilizing primary antibodies to capture the target antigen in one well while leaving the control well colorless, demonstrating the ability to accurately differentiate and measure the protein of interest.
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Recent questions
What is ELISA?
Test to detect and quantify proteins.
How does Direct ELISA work?
Enzyme-linked antibody binds directly to antigen.
What is Indirect ELISA?
Uses primary and secondary antibodies for detection.
Explain Sandwich ELISA.
Two primary antibodies capture the antigen.
How is ELISA used in labs?
Detect and quantify soluble substances like proteins.
Related videos
Summary
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"ELISA: Detecting Proteins with Enzyme Technology"
- ELISA, short for Enzyme-Linked Immunosorbent Assay, is a plate-based technique used to detect and quantify soluble substances like proteins. In ELISA, the antigen is immobilized and detected by enzyme-coupled antibodies, producing a measurable colorful product when a colorless substrate is provided. Different ELISA variants include Direct ELISA, where the enzyme-linked antibody binds directly to the antigen, Indirect ELISA, where a primary and secondary antibody are used, and Sandwich ELISA, where two primary antibodies capture the antigen. In a Sandwich ELISA example, primary antibodies specific for protein X are coated in two wells, one containing the sample with the protein and the other a control. The enzyme-linked antibody binds to the antigen in the sample well, producing a measurable colorful product, while the control well remains colorless.
