What is centrifugation?

Separating particles in suspension based on sedimentation rates.

What is a centrifuge?

Instrument used for high-speed particle separation.

Swinging bucket, non-horizontal, fixed-angle rotors.

Separating particles based on density for purification.

Centrifugation is a vital lab technique that separates particles in suspension based on their sedimentation rates, essential for purification and separation of particles in a liquid medium under a centrifugal field.
Different types of centrifuge rotors, including swinging bucket, non-horizontal, and fixed-angle rotors, are designed for specific rotation speeds, with centrifuge machines consisting of components like a control panel, LED display, protective enclosure, shaft, and motor for sample separation at specified RPM, showcasing the complexity and precision involved in the centrifugation process.

What is centrifugation?
Separating particles in suspension based on sedimentation rates.
What is a centrifuge?
Instrument used for high-speed particle separation.
What is the formula for centrifugal force?
F = m * ω^2 * r.
What are the types of centrifuge rotors?
Swinging bucket, non-horizontal, fixed-angle rotors.
What is the purpose of density gradient centrifugation?
Separating particles based on density for purification.

00:00

Centrifugation is a crucial technique used in labs to separate particles in suspension.
The instrument employed for centrifugation is called a centrifuge, with advanced techniques available for high-speed separation.
The lecture will cover the principles, types, modes, and applications of centrifugation.
Centrifugation is a process that separates particles in suspension based on their sedimentation rates.
The process is essential for the separation, purification, and sedimentation of particles in a liquid medium under a centrifugal field.
The centrifugal force is calculated using the formula F = m * ω^2 * r, where ω is the angular velocity and r is the distance from the center of rotation to the particle.
Relative centrifugal force is a perpendicular force measured relative to Earth's gravity, calculated using the formula RCF = r * rpm^2.
Centrifuge rotors are devices within the centrifuge where sample tubes are placed for separation at desired speeds.
Types of centrifuge rotors include swinging bucket, non-horizontal, and fixed-angle rotors, each designed for specific rotation speeds.
Centrifuge machines consist of components like a control panel, LED display, protective enclosure, shaft, and motor for sample separation at specified RPM.

16:12

Centrifugation process involves different speeds: 1000 at 10000 rpm, 15000, and 25000, to separate particles based on density.
Differential centrifugation is used to prepare sub-cellular fractions by separating particles with varying sedimentation rates.
Density gradient centrifugation separates particles based on density by creating different density gradients, aiding in purification of viruses and separation of particles.
Isopic centrifugation separates particles solely based on density, not size, in a time-independent process, useful for determining particle densities.
Different types of density gradient centrifugation have specific purposes, like isolating micro molecules, cell organelles, and preparing mononuclear cells.
Practical steps in centrifugation involve multiple rounds of centrifugation at varying speeds (e.g., 20000 G, 60000 G) to obtain specific organelles like mitochondria and microsomes.
The process of centrifugation is crucial in labs, with various applications and techniques like differential and density gradient centrifugation, aiding in research and analysis.